[Enhanced luminescence intensity in immunofluorescent preparations following trypsin treatment].
Identifieur interne : 001586 ( Main/Exploration ); précédent : 001585; suivant : 001587[Enhanced luminescence intensity in immunofluorescent preparations following trypsin treatment].
Auteurs : B. Mitov ; M. Aleksandrov ; G K Georgiev ; R. BostandzhievaSource :
- Veterinarno-meditsinski nauki [ 0324-1068 ] ; 1986.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Antigènes viraux.
- pharmacologie : Trypsine.
- Animaux, Bovins, Culture virale, Facteurs temps, Mesures de luminescence, Technique d'immunofluorescence, Température.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Antigens, Viral.
- chemical , pharmacology : Trypsin.
- Animals, Cattle, Fluorescent Antibody Technique, Luminescent Measurements, Temperature, Time Factors, Virus Cultivation.
Abstract
Comparative investigations were carried out on the immunofluorescent preparations of cell cultures infected with bovine viruses--rota-, corona-, respiratory-syncytial, parainfluenza-3, adeno-1, and herpes-1--to test various fixatives and the effect of trypsin in raising the sensitivity of the immunofluorescence method. The effect of trypsin was manifested in fixation with formalin, ethanol, methanol, and acetone treated immunofluorescent preparations of cell cultures infected with rota- and adeno-viruses as well as in fixation with acetone of cultures infected with respiratory syncytial virus, parainfluenza-3 virus, and corona virus. Formalin, ethanol, and partly methanol were shown to be unsuitable for the purpose of fixation of cell culture preparations infected with viruses that contained a lipoprotein envelope. It was found that the treatment of immunofluorescent preparations with trypsin following fixation and prior to their treatment with conjugated antisera enhanced considerably the number of fluorescent cells and the intensity of fluorescence itself provided 0.1 per cent trypsin was used for 5 to 10 min at 37 degrees C--for cell culture preparations, and 0.1 per cent trypsin was used for 20 to 30 min at 37 degrees C--for paraffin sections of acetone-fixed tissues.
PubMed: 3544471
Affiliations:
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Bostandzhieva</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1986">1986</date><idno type="RBID">pubmed:3544471</idno><idno type="pmid">3544471</idno><idno type="wicri:Area/PubMed/Corpus">000748</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000748</idno><idno type="wicri:Area/PubMed/Curation">000748</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000748</idno><idno type="wicri:Area/PubMed/Checkpoint">000737</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000737</idno><idno type="wicri:Area/Ncbi/Merge">000E29</idno><idno type="wicri:Area/Ncbi/Curation">000E29</idno><idno type="wicri:Area/Ncbi/Checkpoint">000E29</idno><idno type="wicri:doubleKey">0324-1068:1986:Mitov B:enhanced:luminescence:intensity</idno><idno type="wicri:Area/Main/Merge">001609</idno><idno type="wicri:Area/Main/Curation">001586</idno><idno type="wicri:Area/Main/Exploration">001586</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Enhanced luminescence intensity in immunofluorescent preparations following trypsin treatment].</title><author><name sortKey="Mitov, B" sort="Mitov, B" uniqKey="Mitov B" first="B" last="Mitov">B. Mitov</name></author><author><name sortKey="Aleksandrov, M" sort="Aleksandrov, M" uniqKey="Aleksandrov M" first="M" last="Aleksandrov">M. Aleksandrov</name></author><author><name sortKey="Georgiev, G K" sort="Georgiev, G K" uniqKey="Georgiev G" first="G K" last="Georgiev">G K Georgiev</name></author><author><name sortKey="Bostandzhieva, R" sort="Bostandzhieva, R" uniqKey="Bostandzhieva R" first="R" last="Bostandzhieva">R. Bostandzhieva</name></author></analytic><series><title level="j">Veterinarno-meditsinski nauki</title><idno type="ISSN">0324-1068</idno><imprint><date when="1986" type="published">1986</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antigens, Viral (analysis)</term><term>Cattle</term><term>Fluorescent Antibody Technique</term><term>Luminescent Measurements</term><term>Temperature</term><term>Time Factors</term><term>Trypsin (pharmacology)</term><term>Virus Cultivation</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Antigènes viraux (analyse)</term><term>Bovins</term><term>Culture virale</term><term>Facteurs temps</term><term>Mesures de luminescence</term><term>Technique d'immunofluorescence</term><term>Température</term><term>Trypsine (pharmacologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Trypsin</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Antigènes viraux</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Trypsine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cattle</term><term>Fluorescent Antibody Technique</term><term>Luminescent Measurements</term><term>Temperature</term><term>Time Factors</term><term>Virus Cultivation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Bovins</term><term>Culture virale</term><term>Facteurs temps</term><term>Mesures de luminescence</term><term>Technique d'immunofluorescence</term><term>Température</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Comparative investigations were carried out on the immunofluorescent preparations of cell cultures infected with bovine viruses--rota-, corona-, respiratory-syncytial, parainfluenza-3, adeno-1, and herpes-1--to test various fixatives and the effect of trypsin in raising the sensitivity of the immunofluorescence method. The effect of trypsin was manifested in fixation with formalin, ethanol, methanol, and acetone treated immunofluorescent preparations of cell cultures infected with rota- and adeno-viruses as well as in fixation with acetone of cultures infected with respiratory syncytial virus, parainfluenza-3 virus, and corona virus. Formalin, ethanol, and partly methanol were shown to be unsuitable for the purpose of fixation of cell culture preparations infected with viruses that contained a lipoprotein envelope. It was found that the treatment of immunofluorescent preparations with trypsin following fixation and prior to their treatment with conjugated antisera enhanced considerably the number of fluorescent cells and the intensity of fluorescence itself provided 0.1 per cent trypsin was used for 5 to 10 min at 37 degrees C--for cell culture preparations, and 0.1 per cent trypsin was used for 20 to 30 min at 37 degrees C--for paraffin sections of acetone-fixed tissues.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Aleksandrov, M" sort="Aleksandrov, M" uniqKey="Aleksandrov M" first="M" last="Aleksandrov">M. Aleksandrov</name><name sortKey="Bostandzhieva, R" sort="Bostandzhieva, R" uniqKey="Bostandzhieva R" first="R" last="Bostandzhieva">R. Bostandzhieva</name><name sortKey="Georgiev, G K" sort="Georgiev, G K" uniqKey="Georgiev G" first="G K" last="Georgiev">G K Georgiev</name><name sortKey="Mitov, B" sort="Mitov, B" uniqKey="Mitov B" first="B" last="Mitov">B. Mitov</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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